Cool LED pE-100 Illumination System

Cool LED

$1,700.00  

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The pE-100 is perfect for your applications where only one excitation colour is required for illuminating your fluorescent stain(s). This source offers intense, stable excitation at a single wavelength.  The user can select from CoolLED's extensive range of LED Wavelengths and it is designed to fit on all current microscopes.  A simple once-ever adjustment will allow optimization to the optical path of your microscope.  The pE-100 is supplied as a complete working package with power supply and control pod.   Manual operation offers instant on/off and 0-100% intensity control.  The control pod accepts a BNC-type external TTL trigger.

There are many applications for CoolLED products in Clinical Screening using fluorescence microscopy.  In fact, fluorescence is used in Pathology, Histopathology, Microbiology, Immunology, Cytology, Histology, Virology, Molecular Biology and Haematology.

The pE-100 illumination systems offer these benefits:

  • Instant On/Off        
  • No warm-up or cool-down
  • Long Lifetime
  • No bulb replacement or alignment
  • Saves Money
  • Fits current and older microscopes*
  • Quiet operation and less heat generated
  • "Green" benefits as uses less energy
  • No Mercury hazard or disposal issues

PLEASE NOTE:  SINCE THIS IS A SINGLE WAVELENGTH SYSTEM, YOU NEED TO PROVIDE US THE WAVELENGTH YOU WILL NEED FOR THE LIGHT SOURCE TO MATCH YOUR FILTER SET.  PLEASE CONTACT US BEFORE PURCHASING.

The chart below offers some of the most used fluorophores and the wavelengths at which they are commonly excited.  It is always wise to check the filter set in your microscope before selecting if you have used this fluorophore previously.

Fluorophore LED Fluorophore LED Fluorophore LED
Acridine                 470                  mCherry                     585*           GFP                           490     
Alexa 488 490 Cy3 530 Hoechst 365
Alexa 488 470* Cy3.5 550 Indo-Cyanine Green 770
Alexa 594 595 Cy5 635 Propidium Iodide 530
Alexa 594 585* Cy7 700 RFP 550
Alexa 660 660 DAPI 365* Rhodamine 530
Auramine 470 DAPI 400 Spectrum Green 530
Calcofluor White 365* Ethidium Bromide 530 Texas Red 565
Calcofluor White 400 FITC 470* TRITC 530
CFP 440 FITC 490 YFP 490
mCherry 565

GFP

470* YFP 500*

 * CoolLED recommended wavelength for that fluorophore based on common filter sets

Wavelength chart

IN ADDITION: THIS LIGHT SOURCE COMES WITH AN ADAPTER RING WHICH IS DETERMINED BY THE SCOPE IT WILL BE USED ON.  PLEASE CONTACT US BEFORE PURCHASE OF THIS LIGHT SOURCE WITH THE SCOPE YOU INTEND TO USE IT ON SO WE CAN PROVIDE THE CORRECT ADAPTER.

 

 

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