AmScope 40x-600x Plan Phase Contrast Culture Fluorescent Inverted Microscope - IN300TA-FL

AmScope 40x-600x Plan Phase Contrast Culture Fluorescent Inverted Microscope - IN300TA-FL

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SKU: IN300TA-FL

Regular price $5,714.99
Regular price $5,955.99 Sale price $5,714.99
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AmScope 40x-600x Plan Phase Contrast Culture Fluorescent Inverted Microscope - IN300TA-FL
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Description

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

 

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

 

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 15X eyepieces expand the maximum magnification to 600X with 8 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

 

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

 

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

 

Phase Contrast

The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

 

Epi-fluorescence Illumination

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

 

Fluorescence

Filters
Filter Set Excitation Filter Emission Filter
B 450-490nm 520nm
G 510-560nm 590nm

 

Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm


Specifications

Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
Ocular Diameter 30mm
Ocular Diopters right ocular
Eyepieces 10X, 15X
Photo-port height-adjustable 23mm tube
Objective Lenses Infinity-corrected
Objective Turret Quadruple
Focusing System
Focus Adjustment Coaxial Coarse/Fine, adjustable tension
Fine Division 0.002mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 172mm x 242mm
Stage X-Y Travel Range 75mm x 40mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 12V 30W halogen
variable intensity
Condenser NA 0.3, iris diaphragm, filter slot
Power 110VAC 60Hz

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan fluor 0.15 28mm 1.5mm
10X plan fluor, phase-contrast 0.30 3mm 1.5mm
20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
40X plan fluor 0.60 2.23mm 1.5mm


Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords

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Shipping

We provide complimentary UPS ground shipping for online orders exceeding $99 to all destinations within the Continental U.S.A. Most items are drop-shipped from one of our suppliers. Due to the highly configurable nature of microscopes and our vast product portfolio, lead times can vary. Should you need expedited shipping, kindly reach out to us for a personalized quote and to ensure your availability.

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Returns

For most items, we accept returns within 14 days of purchase, with the buyer responsible for covering all shipping charges. Please refer to our Full Returns Policy for further details. (Note: Many returns stem from purchasing the wrong product for a specific application. To prevent this, we recommend consulting a Microscope Central expert before making your purchase.

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