AmScope 400X-600X Phase Contrast Inverted Fluorescence Microscope+3.1MP Back-illuminated CMOS Camera

AmScope 400X-600X Phase Contrast Inverted Fluorescence Microscope+3.1MP Back-illuminated CMOS Camera

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SKU: IN480T-FL-3MBI3

Regular price $9,146.99
Regular price $13,170.99 Sale price $9,146.99
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AmScope 400X-600X Phase Contrast Inverted Fluorescence Microscope+3.1MP Back-illuminated CMOS Camera
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Description

The IN480T-FL is our top-of-the-line inverted epi-fluorescence microscope, accommodating diverse observation methods including brightfield, phase-contrast, and epi-fluorescence. The inverted form and modular stage design allow for a wide range of specimen mounts and containers. A wide range of customizations in the Koehler transmitted illumination system provide flexibility and precise control to allow you to achieve optimal results. The reliable and intuitive design ensure you will spend more time focused on your research.

Optics

 

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. Five brightfield objective lenses provide a wide magnification range from 40X to 600X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

 

Viewing Head

 

The viewing head has a 45&deg incline and Siedentopf interpupillary adjustment for flexibility and comfort. The CX series photo-port uses interchangeable camera mounts to support a variety of imaging solutions. The included 23mm tube mount accommodates our huge selection of microscopy cameras with 23mm reduction lenses. Several optional mounting adapters are available for C-mount cameras, including mounts with integrated reduction to optimize field-of-view based on sensor formats. The viewing platform is equipped with a sliding shutter to isolate the light path when using a camera, avoiding stray light from the oculars.

Koehler Transmitted Illumination

 

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. This allows for highly-efficient, optimized illumination for maximum resolution and contrast. The swing-out condenser mount allows the entire condenser assembly to be laterally rotated out of the way for easier specimen placement, and for larger containers. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting for brightfield or for use with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

 

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Camera

The 3.1MP MU313-BI is part of our back-illuminated CMOS camera series. Back-illuminated CMOS sensors provide higher quantum-efficiency by moving the photo-diode layer in front of the electronic components, increasing photon-absorption. This translates to less image-noise and dark-current while maintaining high speeds. The C-mount connection provides flexibility in usage, including C-mount interfaces, reduction lenses, and adapters for most microscope brands.

The included software for Windows, Mac, and Linux provides an imaging solution for photography and videography. The Windows (XP/Vista/7/8/10) version provides an extensive toolset for image-processing and measurements. A lite version of the application is provided for Mac and Linux.

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth
B1 480nm/30nm 505nm 535nm/40nm
Common Stains: Alexa Fluor 488, Cy2, FITC
G1 560nm/40nm 600nm 635nm/60nm
Common Stains: Texas Red, Cy3.5, Mito Tracker Red

 

Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications


Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter 30mm
Eyepieces 10X FN22mm plan wide-field
Photo-port CX-type height-adjustable, 23mm tube
Objective Turret quintuple
Focusing System
Focus Adjustment Coaxial Coarse/Fine, adjustable tension
Fine Division 0.002mm
Coarse Stroke 38mm
Fine Stroke 0.2mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range 120mm x 80mm
Specimen Holders Terasaki plate, Petri dish
Transmitted Illumination 6V 30W halogen
variable intensity
centerable lamp
Condenser NA 0.3, centerable
Condenser Working Distance 72mm maximum
Phase Contrast Annuli 4X/10X and 20X/40X
Power 90-240VAC 50/60Hz wide-band for international use


Camera Specifications

  • Sensor: Sony IMX123 (color)
  • Sensor Type: BSI CMOS
  • Sensor Size: 1/2.8" (6.46mm diagonal)
  • Pixel Size: 2.5µm x 2.5µm
  • Sensor Pixels: 3.1MP (2048x1536)
  • Sensitivity: 600mv
  • Max Framerates: 50fps @ 2048x1536
  • Binning: 1x1
  • Exposure Time: 0.244ms - 15s
  • Connectivity: USB 3.0
  • Compatibility: Windows (32/64 bit) XP/Vista/7/8/10, Mac OSX, Linux
  • Reduction Lens: 0.5X C-mount to 23mm

Contents

 

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair of 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • Camera



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Shipping

We provide complimentary UPS ground shipping for online orders exceeding $99 to all destinations within the Continental U.S.A. Most items are drop-shipped from one of our suppliers. Due to the highly configurable nature of microscopes and our vast product portfolio, lead times can vary. Should you need expedited shipping, kindly reach out to us for a personalized quote and to ensure your availability.

We welcome international orders on a case-by-case basis. Buyers are accountable for any international duties, taxes, brokerage, or import fees that may be incurred. We encourage customers to reach out to us at sales@microscopecentral.com prior to purchase, to ensure the microscope will meet their needs and expectations.

Returns

For most items, we accept returns within 14 days of purchase, with the buyer responsible for covering all shipping charges. Please refer to our Full Returns Policy for further details. (Note: Many returns stem from purchasing the wrong product for a specific application. To prevent this, we recommend consulting a Microscope Central expert before making your purchase.

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