Troubleshooting Common Microscope Issues

Problem Description

Microscope Out-of Focus/Poor Image Quality

Common Issue:

  • The slide is upside down
  • The stage height stop is set too low
  • The coverslip is missing or is too thick/thin
  • A dry objective has oil on it - common with 40x (clean off the oil)
  • No oil or insufficient oil contact when using 100x objective
  • The condenser is too low or the iris is closed

Problem Description

Microscope Does Not Stay In Focus

Common Issue:

  • The slide is not lying flat on the stage
  • The tension adjustment needs to be tightened
  • The nosepiece is not full engaged

Problem Description

Dirt/Dust In Field Of View

Common Issue:

  • If debris moves when you rotate the eyepiece, then the dirt is on the eyepiece
  • If there is a diminished view under one objective, then that objective has debris on it
  • The top of the condenser or lamphouse has debris on it

Problem Description

Camera Not In Focus While Eyepieces Are Focused

Common Issue:

  • Eyepieces not set at zero mark (set them to zero and re-focus)C-Mount focus adjustment is not set properly (focus through eyepieces and then adjust the c-mount focus to match)
  • Make sure you have the proper c-mount for your microscope/camera
  • Make sure the eyepieces are secured

Problem Description

Seeing Double Image/Headache While Using Microscope

Common Issue:

  • Microscope could be out of alignment if bumped or dropped and needs repair
  • The focusing eyepiece is all the way up or down (make sure it is set at zero position)
  • If you need assistance with your microscope, we’re happy to help. Call Microscope Central at 800-219-1451 or email sales@microscopecentral.com.
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