Tips For Proper Use
- Prepare your slide - don’t forget to use a coverslip as microscope optics are corrected for optimal viewing through it.
- Set the IPD to an appropriate distance so that both of your eyes line up with the eyepiece.
- Make sure your focusing eyepiece/eyetube is set to the zero mark, otherwise the microscope may appear to be out of alignment or not parfocal with a camera.
- Move the lowest power objective into place and put the slide on your microscope.
- Use the coarse focus for the low power objective. Close one eye - the one that has the focusing eyepiece/eyetube. If your microscope has two focusing eyepieces/eyetubes, set both to zero and you can use either eye.
- Bring your sample into focus with one eye closed. Once in focus, close that eye and open the other. Use the focusing mechanism on your eyepiece/eyetube to bring the image into focus for this eye. This ensures that the image is in focus for both eyes since your vision is slightly different in each eye.
- Now you can open both of your eyes and observe your specimen in perfect focus.
- Rotate the nosepiece to the next objective.
- Use the fine focus for adjustments as you change objectives. If your microscope is parfocal, you will only need a small turn of the fine focus for each objective (as long as you are selecting them in order of magnification).
- When using the 100x oil objective, apply a small drop of oil to your coverslip and pass the objective through it once or twice to spread the oil and make even contact.
- When finished, switch to your low power objective, remove the slide and be sure to wipe clean any excess oil that is on the front of your 100x oil lens.
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