{"product_id":"optosplit-ii-bp-image-splitter-with-bypass","title":"OptoSplit II BP - Image Splitter with Bypass","description":"\u003cp\u003e\u003cspan\u003eThe Cairn Research OptoSplit II BP emission image splitter is a device that allows a single camera to record images simultaneously at two different optical wavelengths, polarization states, or other differentiated states.  It\u003c\/span\u003e\u003cspan\u003e divides an image into two separate, spatially identical components, displayed side by side on a single sensor - effectively doubling research capacity with just one camera. The BP version adds a convenient single lever \"bypass\" mode ideal for multi-user microscopes, or any setup where simultaneous dual channels are not required for all \u003cimg\u003eimaging.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTraditionally, dual channel imaging is performed using an electronic filter changer. The switching speed of an electronic filter changer is a major limitation to speed and science.  With the OptoSplit, two images are captured in parallel with no time lost to switching filters.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe OptoSplit uses a unique rotating mirror cradle to ensure excellent image registration and features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduce scatter.  Cairn's proprietary lens design supports sensors up to 29.4mm diagonal.  The large aperture design results in improved off-axis correction that maximizes image flatness and minimizes chromatic distortion to give enhanced performance with all sensors.  All optical surfaces feature AR coatings, 425nm to 875nm.  The BP version has a slightly larger footprint with additional space for more auxiliary components.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe OptoSplit II BP is ideal for many scientific applications, including:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimultaneous multi-fluorescent probe imaging\u003c\/li\u003e\n\u003cli\u003eLive cell and high speed imaging\u003c\/li\u003e\n\u003cli\u003eFörster Resonance Energy Transfer (FRET)\u003c\/li\u003e\n\u003cli\u003eRatiometric calcium, voltage \u0026amp; pH imaging\u003c\/li\u003e\n\u003cli\u003ePolarization studies (anisotropy)\u003c\/li\u003e\n\u003cli\u003eSimultaneous fluorescence and transmitted\/DIC\/phase imaging\u003c\/li\u003e\n\u003cli\u003eSimultaneous dual Z depth imaging\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eThe OptoSplit II BP is simple to add to most microscopes, and is compatible with:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpinning disk confocal microscopy\u003c\/li\u003e\n\u003cli\u003eTotal Internal Reflection Fluorescence (TIRF)\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eLight sheet and Single Plane Illumination Microscopy (SPIM)\u003c\/li\u003e\n\u003cli\u003e3D super resolution PALM\/STORM (using cylindrical lenses)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eImaging with the OptoSplit does not require any custom camera drivers.  However, many software packages include useful tools to assist with registration and analysis.  An OptoSplit plugin is also available in ImageJ (Fiji) and MicroManager.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis OptoSplit II BP package includes the following:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eC-mount input and output connections (22mm FOV)\u003c\/li\u003e\n\u003cli\u003e1x magnification optics\u003c\/li\u003e\n\u003cli\u003eRectangular input diaphragm\u003c\/li\u003e\n\u003cli\u003e1 Empty filter cube (requires 25mm emission filters, 26x38x2mm dichroic)\u003c\/li\u003e\n\u003cli\u003eCalibration cube\u003c\/li\u003e\n\u003cli\u003e1 OptoSplit support jack\u003c\/li\u003e\n\u003cli\u003eShutter plate\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eSingle lever switching between split mode and bypass mode\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFilters not included.  Alternate adapters are available for larger field-of-view, such as F-Mount or T-Mount.  1.3x \u003cspan style=\"font-size: 0.875rem;\"\u003emagnification optics are available upon request.  Contact us for polarization options including a beamsplitter cube and rotatable polarizer, or multifocal optics.  Additional filter cubes and drop-in 25mm holders are available.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eWe are happy to recommend and supply filter sets.  Please contact us to discuss your application.\u003c\/em\u003e\u003c\/p\u003e","brand":"Cairn Research","offers":[{"title":"Default Title","offer_id":53212211118377,"sku":"P280\/210\/000-0BP-BUNDLE","price":19560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0452\/4209\/files\/OptoSplit-II-ByPass-Product-1.jpg?v=1779913658","url":"https:\/\/microscopecentral.com\/products\/optosplit-ii-bp-image-splitter-with-bypass","provider":"Microscope Central","version":"1.0","type":"link"}